lipofectamine 3000 (Thermo Fisher)
Structured Review
Lipofectamine 3000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine+3000/Lapatinib/pmc13273694-118-18-20
Average 94 stars, based on 1 article reviews
Images
Related Articles
Plasmid Preparation:Article Title: Transition of CELF2 PAS usage promotes recovery of refractory JDM through alternative splicing regulation of CTSB Article Snippet: Then validated products were digested with Spe I/Not I sites and cloned into pIS1 vector (Addgene, USA). .. 2 μg plasmid was transfected into SK-N-BE(2) cells using Article Title: Antisense oligonucleotides targeting the SARS-CoV-2 nucleocapsid gene decrease viral titers in hamsters Article Snippet: .. In short, SARS-CoV-2/Zsgreen was obtained from a pCC1-4K-SARS-CoV-2-ZsGreen plasmid in VeroE6 cells, which were transfected using Article Title: A critical signaling role for diacylglycerol in phagocytosis of M. tuberculosis Article Snippet: Plasmids were extracted from bacterial cultures using Omega E.Z.N.Z Plasmid DNA Mini Kit miniprep kit (Omega Bio-Tek, product D6945-02) following manufacturer instructions. .. Replication deficient lentivirus was produced in HEK 293T cells (ATCC, product CRL-3216) by transfection of the complete lentiCRISPRv2 plasmid, psPAX (a gift from Didier Trono, Addgene plasmid #12260), and pMD2.G (a gift from Didier Trono, Addgene plasmid #12259) via Article Title: Egr-1 promotes the proliferation and migration of vascular smooth muscle cells by transcriptionally activating Egr-2 in arteriovenous fistulas Article Snippet: Rat EGR1 promoter (~1.3 Kb; PCR-amplified from rat VSMCs) cloned into pGL3-basic luciferase rep plasmid (Promega Corporation) to form EGR1 luciferase reporters. .. Briefly, VSMCs were (3×10 4 /well in 24-well plates) seeded and incubated for 24 h. Specific plasmids, lentivirus and 1.5 ng of pRL-TK Renilla plasmid were transfected with Transfection:Article Title: Transition of CELF2 PAS usage promotes recovery of refractory JDM through alternative splicing regulation of CTSB Article Snippet: Then validated products were digested with Spe I/Not I sites and cloned into pIS1 vector (Addgene, USA). .. 2 μg plasmid was transfected into SK-N-BE(2) cells using Article Title: Mutant p53 epigenetically rewires CXCL10 to promote CD8⁺ T-cell infiltration and enhance the anti-PD-1 response in advanced prostate cancer Article Snippet: Fluorescence images were acquired with a fluorescence microscope (Olympus IX73, Olympus Corporation, Tokyo, Japan), and the number of EdU-positive proliferating cells was quantified for statistical analysis. .. RM-1 cells were transfected with Trp53 -specific siRNA (sense: 5′-GAAUGAGGCCUUAGAGUUATT-3′; antisense: 5′-UAACUCUAAGGCCUCAUUCTT-3′) via Article Title: Characterization and functional analysis of BRCA1 and BRCA2 variants in a cohort of 100 unselected patients undergoing germline screening. Article Snippet: .. Transient transfection was performed on both MCF7 and MDA-MB-231 cells using Article Title: Antisense oligonucleotides targeting the SARS-CoV-2 nucleocapsid gene decrease viral titers in hamsters Article Snippet: .. In short, SARS-CoV-2/Zsgreen was obtained from a pCC1-4K-SARS-CoV-2-ZsGreen plasmid in VeroE6 cells, which were transfected using Article Title: A critical signaling role for diacylglycerol in phagocytosis of M. tuberculosis Article Snippet: Plasmids were extracted from bacterial cultures using Omega E.Z.N.Z Plasmid DNA Mini Kit miniprep kit (Omega Bio-Tek, product D6945-02) following manufacturer instructions. .. Replication deficient lentivirus was produced in HEK 293T cells (ATCC, product CRL-3216) by transfection of the complete lentiCRISPRv2 plasmid, psPAX (a gift from Didier Trono, Addgene plasmid #12260), and pMD2.G (a gift from Didier Trono, Addgene plasmid #12259) via Article Title: Egr-1 promotes the proliferation and migration of vascular smooth muscle cells by transcriptionally activating Egr-2 in arteriovenous fistulas Article Snippet: Rat EGR1 promoter (~1.3 Kb; PCR-amplified from rat VSMCs) cloned into pGL3-basic luciferase rep plasmid (Promega Corporation) to form EGR1 luciferase reporters. .. Briefly, VSMCs were (3×10 4 /well in 24-well plates) seeded and incubated for 24 h. Specific plasmids, lentivirus and 1.5 ng of pRL-TK Renilla plasmid were transfected with Article Title: Lapatinib induces ferroptosis in osteosarcoma via the SLC1A5-GPX4 axis Article Snippet: .. Cells were seeded in 6-well plates (5 × 10 5 cells/well) and transfected with 50 nM siRNA using Multiple Displacement Amplification:Article Title: Characterization and functional analysis of BRCA1 and BRCA2 variants in a cohort of 100 unselected patients undergoing germline screening. Article Snippet: .. Transient transfection was performed on both MCF7 and MDA-MB-231 cells using Produced:Article Title: A critical signaling role for diacylglycerol in phagocytosis of M. tuberculosis Article Snippet: Plasmids were extracted from bacterial cultures using Omega E.Z.N.Z Plasmid DNA Mini Kit miniprep kit (Omega Bio-Tek, product D6945-02) following manufacturer instructions. .. Replication deficient lentivirus was produced in HEK 293T cells (ATCC, product CRL-3216) by transfection of the complete lentiCRISPRv2 plasmid, psPAX (a gift from Didier Trono, Addgene plasmid #12260), and pMD2.G (a gift from Didier Trono, Addgene plasmid #12259) via Article Title: YTHDF2 regulates the adriamycin (ADM) resistance in NKTCL by modulating the m6A modification of SLC16A9 Article Snippet: .. Lentiviral particles were produced by co-transfecting the expression vectors with packaging and envelope plasmids into 293T cells using Incubation:Article Title: Egr-1 promotes the proliferation and migration of vascular smooth muscle cells by transcriptionally activating Egr-2 in arteriovenous fistulas Article Snippet: Rat EGR1 promoter (~1.3 Kb; PCR-amplified from rat VSMCs) cloned into pGL3-basic luciferase rep plasmid (Promega Corporation) to form EGR1 luciferase reporters. .. Briefly, VSMCs were (3×10 4 /well in 24-well plates) seeded and incubated for 24 h. Specific plasmids, lentivirus and 1.5 ng of pRL-TK Renilla plasmid were transfected with Knockdown:Article Title: Lapatinib induces ferroptosis in osteosarcoma via the SLC1A5-GPX4 axis Article Snippet: .. Cells were seeded in 6-well plates (5 × 10 5 cells/well) and transfected with 50 nM siRNA using Western Blot:Article Title: Lapatinib induces ferroptosis in osteosarcoma via the SLC1A5-GPX4 axis Article Snippet: .. Cells were seeded in 6-well plates (5 × 10 5 cells/well) and transfected with 50 nM siRNA using Expressing:Article Title: YTHDF2 regulates the adriamycin (ADM) resistance in NKTCL by modulating the m6A modification of SLC16A9 Article Snippet: .. Lentiviral particles were produced by co-transfecting the expression vectors with packaging and envelope plasmids into 293T cells using |
